Page 673 - 先天性心脏病的导管术：从婴幼儿期到成人期

908
A. Christensen et al.
52.9 Postprocedural Care
The samples can be removed from the bioptome using a sterile
needle and should be placed on sterile gauze as appropriate for
transport to the laboratory. The type of storage depends on the
clinical question [4]. Some standard fixatives and techniques of
52 心内膜心肌活检 655
analysis are listed in Table 52.2.
Table 52.2 Some standard fixatives and techniques of analysis for common
表 52.2 针对常见临床问题的一些标准固定剂和分析技术。
clinical questions
Fixative Technique Clinical question
10% neutral-buffered Light microscopy Transplant rejection
formalin at room Myocarditis
temperature Infiltrative/
unexplained
cardiomyopathy
Tumours
Immunohistochemistry Transplant rejection
Tumours
Polymerase chain reaction Myocarditis
4% glutaraldehyde at Transmission electron Unexplained
room temperature microscopy cardiomyopathy
(amyloidosis,
glycogen storage
diseases, lysosomal
storage diseases or
mitochondrial
disease)
Anthracycline-
induced cardiotoxicity
Flash-frozen tissue Culture, polymerase chain Myocarditis
transported on ice or reaction (PCR), reverse
fixative like RNA later transcriptase PCR
(Ambion, Austin, TX) (rtPCR)
or snap frozen in Immunofluorescence Storage diseases
OCT-embedding Immunohistochemistry Tumour typing
medium or liquid Molecular studies Amyloid classification
nitrogen
Muscular dystrophies

致谢

我们感谢我们的技术专家 Dariusz Mroczek 和 Renato Prosio 提供图像。

参考文献

[1] Wagner K, Oliver MC, Boyle GJ, et al. Endomyocardial biopsy in pediatric heart trans-

plant recipients: a useful exercise? (Analysis of 1169 biopsies). Pediatr Transplant.
2000;4(3):186-92. https://doi.org/10.1034/j.1399-3046.2000.00100.x.
[2] Peng DM, Ding VY, Hollander SA, et al. Long-term surveillance biopsy: is it neces-
sary after pediatric heart transplant? Pediatr Transplant. 2019;23(1):1-9. https://doi.
org/10.1111/petr.13330.
[3] Narula N, Narula J, Dec GW. Endomyocardial biopsy for non-transplant-related disorders.